A simple, accurate and precise dual wavelength spectrophotometric method was developed for simultaneous determination of Dexketoprofen Tromatamol and Dicyclomine HCl in laboratory binary mixture. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The wavelengths selected for determination of Dexketoprofen Tromatamol were 205 nm and 211 nm, whereas, the wavelengths selected for determination of Dicyclomine HCl were 239 nm and 287 nm. Dist. Water was taken as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 9-24 μg/ml for Dexketoprofen Tromatamol and 10-60 μg/ml for Dicyclomine HCl. Accuracy of method was found between 98‐102 percent. The precision (intra‐day, inter‐day and repeatability) of method was found within limits. The proposed method was successfully applied to determination of these drugs in mixture.
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